5 December 2012 In vivo observation of age-related structural changes of dermal collagen in human facial skin using collagen-sensitive second harmonic generation microscope equipped with 1250-nm mode-locked Cr:Forsterite laser
Author Affiliations +
Abstract
In vivo visualization of human skin aging is demonstrated using a Cr:Forsterite (Cr:F) laser-based, collagen-sensitive second harmonic generation (SHG) microscope. The deep penetration into human skin, as well as the specific sensitivity to collagen molecules, achieved by this microscope enables us to clearly visualize age-related structural changes of collagen fiber in the reticular dermis. Here we investigated intrinsic aging and/or photoaging in the male facial skin. Young subjects show dense distributions of thin collagen fibers, whereas elderly subjects show coarse distributions of thick collagen fibers. Furthermore, a comparison of SHG images between young and elderly subjects with and without a recent life history of excessive sun exposure show that a combination of photoaging with intrinsic aging significantly accelerates skin aging. We also perform image analysis based on two-dimensional Fourier transformation of the SHG images and extracted an aging parameter for human skin. The in vivo collagen-sensitive SHG microscope will be a powerful tool in fields such as cosmeceutical sciences and anti-aging dermatology.
© The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Takeshi Yasui, Takeshi Yasui, Makoto Yonetsu, Makoto Yonetsu, Ryosuke Tanaka, Ryosuke Tanaka, Yuji Tanaka, Yuji Tanaka, Shu-ichiro Fukushima, Shu-ichiro Fukushima, Toyonobu Yamashita, Toyonobu Yamashita, Yuki Ogura, Yuki Ogura, Tetsuji Hirao, Tetsuji Hirao, Hiroyuki Murota, Hiroyuki Murota, Tsutomu Araki, Tsutomu Araki, } "In vivo observation of age-related structural changes of dermal collagen in human facial skin using collagen-sensitive second harmonic generation microscope equipped with 1250-nm mode-locked Cr:Forsterite laser," Journal of Biomedical Optics 18(3), 031108 (5 December 2012). https://doi.org/10.1117/1.JBO.18.3.031108 . Submission:
JOURNAL ARTICLE
11 PAGES


SHARE
Back to Top