20 March 2013 Spectroscopic imaging of serum proteins using quantum cascade lasers
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Abstract
First measurements of biomedical imaging using quantum cascade lasers (QCL) are presented. We report spectroscopic imaging of serum proteins using QCLs as an example for monitoring surface biocontamination. We found that dry smears of human serum can be spectroscopically imaged, identified, and quantified with high sensitivity and specificity. The core parts of the imaging platform consist of optically multiplexing three QCLs and an uncooled microbolometer camera. We show imaging of human serum proteins at 6.1, 9.25, and 9.5 μm QCLs with high sensitivity and specificity. The sensitivity limit of 3  μg/cm 2 of the human serum spot was measured at an S/N=3 .The specificity of human serum detection was measured at 99% probability at a threshold of 77  μg/cm 2 . We anticipate our imaging technique to be a starting point for more sophisticated biomolecular diagnostic applications.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
Anadi Mukherjee, Anadi Mukherjee, Quentin Bylund, Quentin Bylund, Manu Prasanna, Manu Prasanna, Yotam Margalit, Yotam Margalit, Tarik Tihan, Tarik Tihan, } "Spectroscopic imaging of serum proteins using quantum cascade lasers," Journal of Biomedical Optics 18(3), 036011 (20 March 2013). https://doi.org/10.1117/1.JBO.18.3.036011 . Submission:
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