Widefield quantitative multiplex surface enhanced Raman scattering imaging in vivo

Patrick McVeigh; Rupananda J. Mallia; Israel Veilleux; Brian C. Wilson

doi:10.1117/1.JBO.18.4.046011

16 April 2013 Widefield quantitative multiplex surface enhanced Raman scattering imaging in vivo

Patrick McVeigh, Rupananda J. Mallia, Israel Veilleux, Brian C. Wilson

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Journal of Biomedical Optics, Vol. 18, Issue 4, 046011 (April 2013). https://doi.org/10.1117/1.JBO.18.4.046011

Abstract

In recent years numerous studies have shown the potential advantages of molecular imaging in vitro and in vivo using contrast agents based on surface enhanced Raman scattering (SERS), however the low throughput of traditional point-scanned imaging methodologies have limited their use in biological imaging. In this work we demonstrate that direct widefield Raman imaging based on a tunable filter is capable of quantitative multiplex SERS imaging in vivo, and that this imaging is possible with acquisition times which are orders of magnitude lower than achievable with comparable point-scanned methodologies. The system, designed for small animal imaging, has a linear response from (0.01 to 100 pM), acquires typical in vivo images in <10 s , and with suitable SERS reporter molecules is capable of multiplex imaging without compensation for spectral overlap. To demonstrate the utility of widefield Raman imaging in biological applications, we show quantitative imaging of four simultaneous SERS reporter molecules in vivo with resulting probe quantification that is in excellent agreement with known quantities (R² <0.98 ).

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Patrick McVeigh, Rupananda J. Mallia, Israel Veilleux, and Brian C. Wilson "Widefield quantitative multiplex surface enhanced Raman scattering imaging in vivo," Journal of Biomedical Optics 18(4), 046011 (16 April 2013). https://doi.org/10.1117/1.JBO.18.4.046011

Published: 16 April 2013

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