3 June 2013 Video-rate imaging of microcirculation with single-exposure oblique back-illumination microscopy
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Abstract
Oblique back-illumination microscopy (OBM) is a new technique for simultaneous, independent measurements of phase gradients and absorption in thick scattering tissues based on widefield imaging. To date, OBM has been used with sequential camera exposures, which reduces temporal resolution, and can produce motion artifacts in dynamic samples. Here, a variation of OBM that allows single-exposure operation with wavelength multiplexing and image splitting with a Wollaston prism is introduced. Asymmetric anamorphic distortion induced by the prism is characterized and corrected in real time using a graphics-processing unit. To demonstrate the capacity of single-exposure OBM to perform artifact-free imaging of blood flow, video-rate movies of microcirculation
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
Tim N. Ford, Jerome Mertz, "Video-rate imaging of microcirculation with single-exposure oblique back-illumination microscopy," Journal of Biomedical Optics 18(6), 066007 (3 June 2013). https://doi.org/10.1117/1.JBO.18.6.066007
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