2 August 2013 Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels
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Abstract
The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines of different metastatic ability are investigated by fluorescence spectroscopy. The normal (fibroblast), moderately metastatic (DU-145), and advanced metastatic (PC-3) cell lines were each selectively excited at 300 nm, and their fluorescence emission spectra are analyzed using principal component analysis to explore the differences of the relative contents of tryptophan and reduced nicotinamide adenine dinucleotide in these cell lines. The results show that the tryptophan emission featured predominantly in the fluorescence spectra of the advanced metastatic cancer cells in comparison with the moderately metastatic cancer and normal cells.
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
Yang Pu, Yang Pu, Jianpeng Xue, Jianpeng Xue, Wubao Wang, Wubao Wang, Baogang Xu, Baogang Xu, Yueqing Gu, Yueqing Gu, Rui Tang, Rui Tang, Ellen Ackerstaff, Ellen Ackerstaff, Jason A. Koutcher, Jason A. Koutcher, Samuel Achilefu, Samuel Achilefu, Robert R. Alfano, Robert R. Alfano, } "Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels," Journal of Biomedical Optics 18(8), 087002 (2 August 2013). https://doi.org/10.1117/1.JBO.18.8.087002 . Submission:
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