25 September 2013 Impact of treatment response metrics on photodynamic therapy planning and outcomes in a three-dimensional model of ovarian cancer
Author Affiliations +
Abstract
Common methods to characterize treatment efficacy based on morphological imaging may misrepresent outcomes and exclude effective therapies. Using a three-dimensional model of ovarian cancer, two functional treatment response metrics are used to evaluate photodynamic therapy (PDT) efficacy: total volume, calculated from viable and nonviable cells, and live volume, calculated from viable cells. The utility of these volume-based metrics is corroborated using independent reporters of photodynamic activity: viability, a common fluorescence-based ratiometric analysis, and photosensitizer photobleaching, which is characterized by a loss of fluorescence due in part to the production of reactive species during PDT. Live volume correlated with both photobleaching and viability, suggesting that it was a better reporter of PDT efficacy than total volume, which did not correlate with either metric. Based on these findings, live volume and viability are used to probe the susceptibilities of tumor populations to a range of PDT dose parameters administered using 0.25, 1, and 10
© 2013 Society of Photo-Optical Instrumentation Engineers (SPIE)
Sriram R. Anbil, Sriram R. Anbil, Imran Rizvi, Imran Rizvi, Jonathan P. Celli, Jonathan P. Celli, Nermina Alagic, Nermina Alagic, Brian W. Pogue, Brian W. Pogue, Tayyaba Hasan, Tayyaba Hasan, } "Impact of treatment response metrics on photodynamic therapy planning and outcomes in a three-dimensional model of ovarian cancer," Journal of Biomedical Optics 18(9), 098004 (25 September 2013). https://doi.org/10.1117/1.JBO.18.9.098004 . Submission:
JOURNAL ARTICLE
14 PAGES


SHARE
Back to Top