26 August 2013 Lipid droplet pattern and nondroplet-like structure in two fat/i] mutants of Caenorhabditis elegans revealed by coherent anti-Stokes Raman scattering microscopy
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Abstract
Lipid is an important energy source and essential component for plasma and organelle membranes in all kinds of cells. Coherent anti-Stokes Raman scattering (CARS) microscopy is a label-free and nonlinear optical technique that can be used to monitor the lipid distribution in live organisms. Here, we utilize CARS microscopy to investigate the pattern of lipid droplets in two live Caenorhabditis elegans mutants (fat-2 and fat-3). The CARS images showed a striking decrease in the size, number, and content of lipid droplets in the fat-2 mutant but a slight difference in the fat-3 mutant as compared with the wild-type worm. Moreover, a nondroplet-like structure with enhanced CARS signal was detected for the first time in the uterus of fat-2 and fat-3 mutants. In addition, transgenic fat-2 mutant expressing a GFP fusion protein of vitellogenin-2 (a yolk lipoprotein) revealed that the enhanced CARS signal colocalized with the GFP signal, which suggests that the nondroplet-like structure is primarily due to the accumulation of yolk lipoproteins. Together, this study implies that CARS microscopy is a potential tool to study the distribution of yolk lipoproteins, in addition to lipid droplets, in live animals.
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Yung-Hsiang Yi, Cheng-Hao Chien, Wei-Wen Chen, Tian-Hsiang Ma, Kuan-Yu Liu, Yu-Sun Chang, Ta-Chau Chang, Szecheng J. Lo, "Lipid droplet pattern and nondroplet-like structure in two fat/i] mutants of Caenorhabditis elegans revealed by coherent anti-Stokes Raman scattering microscopy," Journal of Biomedical Optics 19(1), 011011 (26 August 2013). https://doi.org/10.1117/1.JBO.19.1.011011 . Submission:
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