20 September 2013 Rapid detection of urinary polyomavirus BK by heterodyne-based surface plasmon resonance biosensor
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In renal transplant patients, immunosuppressive therapy may result in the reactivation of polyomavirus BK (BKV), leading to polyomavirus-associated nephropathy (PVAN), which inevitably causes allograft failure. Since the treatment outcomes of PVAN remain unsatisfactory, early identification and continuous monitoring of BKV reactivation and reduction of immunosuppressants are essential to prevent PVAN development. The present study demonstrated that the developed dual-channel heterodyne-based surface plasmon resonance (SPR) biosensor is applicable for the rapid detection of urinary BKV. The use of a symmetrical reference channel integrated with the poly(ethylene glycol)-based low-fouling self-assembled monolayer to reduce the environmental variations and the nonspecific noise was proven to enhance the sensitivity in urinary BKV detection. Experimentally, the detection limit of the biosensor for BKV detection was estimated to be around 8500  copies/mL . In addition, urine samples from five renal transplant patients were tested to rapidly distinguish PVAN-positive and PVAN-negative renal transplant patients. By virtue of its simplicity, rapidity, and applicability, the SPR biosensor is a remarkable potential to be used for continuous clinical monitoring of BKV reactivation.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Li-Chen Su, Li-Chen Su, Ya-Chung Tian, Ya-Chung Tian, Ying-Feng Chang, Ying-Feng Chang, Chien Chou, Chien Chou, Chao-Sung Lai, Chao-Sung Lai, } "Rapid detection of urinary polyomavirus BK by heterodyne-based surface plasmon resonance biosensor," Journal of Biomedical Optics 19(1), 011013 (20 September 2013). https://doi.org/10.1117/1.JBO.19.1.011013 . Submission:

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