24 January 2014 Electric field Monte Carlo simulation of focused stimulated emission depletion beam, radially and azimuthally polarized beams for in vivo deep bioimaging
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J. of Biomedical Optics, 19(1), 011022 (2014). doi:10.1117/1.JBO.19.1.011022
Abstract
An electric field Monte Carlo method is used to study the focal spot of a stimulated emission depletion (STED) beam, radially and azimuthally polarized beams in a turbid medium as a function of the scattering coefficient. To consider the diffraction of light of the wave nature, the wavefront is decomposed into a set of secondary spherical subwaves according to the Huygens principle. From the simulation results, we can find that the STED beam can still form a doughnut focal spot inside the turbid medium. These simulation results are important for the feasibility study of STED microscopy for in vivo deep bioimaging. Similarly, the focal spot for an azimuthally polarized beam can also keep a doughnut spot at the focal plane in a turbid medium.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Fuhong Cai, Sailing He, "Electric field Monte Carlo simulation of focused stimulated emission depletion beam, radially and azimuthally polarized beams for in vivo deep bioimaging," Journal of Biomedical Optics 19(1), 011022 (24 January 2014). http://dx.doi.org/10.1117/1.JBO.19.1.011022
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KEYWORDS
Monte Carlo methods

Stimulated emission depletion microscopy

Scattering

Laser scattering

Spherical lenses

Microscopy

Optical simulations

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