29 January 2014 Intrinsic photosensitizer fluorescence measured using multi-diameter single-fiber spectroscopy in vivo
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J. of Biomedical Optics, 19(1), 015010 (2014). doi:10.1117/1.JBO.19.1.015010
Abstract
Quantification of fluorescence in vivo is complicated by the influence of tissue optical properties on the collected fluorescence signal. When tissue optical properties in the measurement volume are quantified, one can obtain the intrinsic fluorescence, which equals the product of fluorophore absorption coefficient and quantum yield. We applied this method to in vivo single-fiber fluorescence spectroscopy measurements on mouse tongue, skin, liver, and oral squamous cell carcinoma, where we detected intrinsic fluorescence spectra of the photosensitizers chlorin e6 and Bremachlorin at t=[3,4.5,6,24,48]  h incubation time. We observed a tissue-dependent maximum of 35% variation in the total correction factor over the visible wavelength range. Significant differences in spectral shape over time between sensitizers were observed. Although the wavelength position of the fluorescence intensity maximum for ce6 shifted to the red, Bremachlorin showed a blue shift. Furthermore, the Bremachlorin peak appeared to be broader than the ce6 fluorescence peak. Intrinsic fluorescence intensity, which can be related to photosensitizer concentration, was decreasing for all time points but showed significantly more Bremachlorin present compared to ce6 at long incubation times. Results from this study can be used to define an optimal treatment protocol for Bremachlorin-based photodynamic therapy.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Floor van Leeuwen-van Zaane, Ute A. Gamm, Pieter B. van Driel, Thomas J. Snoeks, Henriette S. de Brujin, Angélique van der Ploeg-van den Heuvel, Henricus J. C. M. Sterenborg, Clemens W. Lowik, Arjen Amelink, Dominic J. Robinson, "Intrinsic photosensitizer fluorescence measured using multi-diameter single-fiber spectroscopy in vivo," Journal of Biomedical Optics 19(1), 015010 (29 January 2014). http://dx.doi.org/10.1117/1.JBO.19.1.015010
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KEYWORDS
Luminescence

Tissues

Tumors

Tissue optics

Absorption

In vivo imaging

Fluorescence spectroscopy

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