27 January 2014 Multiplexed label-free optical biosensor for medical diagnostics
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Abstract
This paper describes a new multiplexed label-free biosensor. The detection technology is based on nanostructured gold-polymer surfaces. These surfaces support surface plasmon resonance modes that can be probed by a miniaturized optical setup. The optical characterization of the sensing chip shows the sensitivity and the limit-of-detection to refractive index changes. Moreover, by studying the progressive adhesion of molecular monolayers of polyelectrolytes, the decay of the plasmonic mode electric field above the surface has been reconstructed. A multiplexed label-free biosensing device is then described and characterized in terms of sensitivity, lateral resolution, and sensitivity to a model biological assay. The sensitivity in imaging mode of the device is of the order of 10 −6 refractive index units, while the measured lateral resolution is 6.25 μm within a field of view of several tenths of mm 2 , making the instrument unique in terms of multiplexing capability. Finally, the proof-of-concept application of the technology as a point-of-care diagnostic tool for an inflammatory marker is demonstrated.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Barbara Bottazzi, Barbara Bottazzi, Lucia Fornasari, Lucia Fornasari, Ana Frangolho, Ana Frangolho, Silvia Giudicatti, Silvia Giudicatti, Alberto Mantovani, Alberto Mantovani, Franco Marabelli, Franco Marabelli, Gerardo Marchesini, Gerardo Marchesini, Paola Pellacani, Paola Pellacani, Rita Therisod, Rita Therisod, Andrea Valsesia, Andrea Valsesia, } "Multiplexed label-free optical biosensor for medical diagnostics," Journal of Biomedical Optics 19(1), 017006 (27 January 2014). https://doi.org/10.1117/1.JBO.19.1.017006 . Submission:
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