1 October 2014 Vertically scanned laser sheet microscopy
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Laser sheet microscopy is a widely used imaging technique for imaging the three-dimensional distribution of a fluorescence signal in fixed tissue or small organisms. In laser sheet microscopy, the stripe artifacts caused by high absorption or high scattering structures are very common, greatly affecting image quality. To solve this problem, we report here a two-step procedure which consists of continuously acquiring laser sheet images while vertically displacing the sample, and then using the variational stationary noise remover (VSNR) method to further reduce the remaining stripes. Images from a cleared murine colon acquired with a vertical scan are compared with common stitching procedures demonstrating that vertically scanned light sheet microscopy greatly improves the performance of current light sheet microscopy approaches without the need for complex changes to the imaging setup and allows imaging of elongated samples, extending the field of view in the vertical direction.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Di Dong, Di Dong, Alicia Arranz, Alicia Arranz, Shouping Zhu, Shouping Zhu, Yujie Yang, Yujie Yang, Liangliang Shi, Liangliang Shi, Jun Wang, Jun Wang, Chen Shen, Chen Shen, Jie Tian, Jie Tian, Jorge Ripoll, Jorge Ripoll, } "Vertically scanned laser sheet microscopy," Journal of Biomedical Optics 19(10), 106001 (1 October 2014). https://doi.org/10.1117/1.JBO.19.10.106001 . Submission:

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