27 June 2014 Surface plasmon resonance–based infrared biosensor for cell studies with simultaneous control
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Abstract
We report a label-free infrared surface plasmon biosensor with a double-chamber flow cell for continuous monitoring of morphological changes in cell culture exposed to various stimuli. In this technique, the monolayer of cultured cells is divided into two halves by a barrier, allowing the treatment of one half while the other serves as control. We demonstrate the advantages of this setup in test experiments that track kinetics of the IEC-18 cell layer response to variations in extracellular Ca2+ concentration. The sensitivity of the presented method was found to be an order of magnitude higher compared to the single-chamber biosensor.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Alexander Zilbershtein, Amir Bein, Vladislav G. Lirtsman, Betty Schwartz, Michael Golosovsky, Dan Davidov, "Surface plasmon resonance–based infrared biosensor for cell studies with simultaneous control," Journal of Biomedical Optics 19(11), 111608 (27 June 2014). https://doi.org/10.1117/1.JBO.19.11.111608 . Submission:
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