27 June 2014 Surface plasmon resonance–based infrared biosensor for cell studies with simultaneous control
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Abstract
We report a label-free infrared surface plasmon biosensor with a double-chamber flow cell for continuous monitoring of morphological changes in cell culture exposed to various stimuli. In this technique, the monolayer of cultured cells is divided into two halves by a barrier, allowing the treatment of one half while the other serves as control. We demonstrate the advantages of this setup in test experiments that track kinetics of the IEC-18 cell layer response to variations in extracellular Ca2+ concentration. The sensitivity of the presented method was found to be an order of magnitude higher compared to the single-chamber biosensor.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Alexander Zilbershtein, Alexander Zilbershtein, Amir Bein, Amir Bein, Vladislav G. Lirtsman, Vladislav G. Lirtsman, Betty Schwartz, Betty Schwartz, Michael Golosovsky, Michael Golosovsky, Dan Davidov, Dan Davidov, } "Surface plasmon resonance–based infrared biosensor for cell studies with simultaneous control," Journal of Biomedical Optics 19(11), 111608 (27 June 2014). https://doi.org/10.1117/1.JBO.19.11.111608 . Submission:
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