Probing single-tumor cell interactions with different-age type I collagen networks by synchrotron-based Fourier transform infrared microspectroscopy

Abstract. We report here on a first study using synchrotron radiation-based Fourier transform infrared microspectroscopy and imaging to investigate HT1080 human fibrosarcoma cells grown onto different-aged type I collagen networks. Spectral images were analyzed with k-means and fuzzy C-means (FCM) clustering algorithms. K-means delineated tumor cells from their surrounding collagen networks and the latter as a function of age mainly due to specific changes in the sugar absorption region. The FCM analysis gave a better nuance of the spectral images. A progression of the biochemical information was observed upon going from the cellular compartments to the pericellular contact regions and to the intact collagens of the different age groups. Two spectral markers based on sugar and protein bands via the intensity ratio (I1032/I1655) and band area ratio (Asugar/Aamide II), showed an increase in advanced glycation endproducts (AGEs) with age. A clear-separation of the three age groups was obtained for spectra originating from the peripheral contact areas mainly due to changes in protein band intensities. The above-described markers decreased to constant levels for the three conditions indicating a masking of the biochemical information. These results hold promises to better understand the impact of age on tumor progression processes while highlighting new markers of the tumor cell invasion front.

Probing single-tumor cell interactions with different-age type I collagen networks by synchrotron-based Fourier transform infrared microspectroscopy Marie Guilbert, a,b, † Christophe Eklouh-Molinier, a,b, † Katia Wehbe, c Josep Sulé-Suso, d,e Ying Yang, d Gianfelice Cinque, c Pierre Jeannesson, a,b and Ganesh D. Sockalingum a,b, * 1 Introduction Studying individual cancer cells represents an increasing area of major interest in the understanding of mechanisms implicated in tumor progression. However, investigating tumor cell migration requires considering the cell microenvironment since in vivo, cells develop specific interactions with their counterparts and with extracellular matrix (ECM) proteins to promote cell adhesion, proliferation, and migration. 1 Most studies are performed in classical cell culture systems where cells are seeded on plane and rigid substrates, such as plastic or glass, which do not take into account the matrix components. Dynamic interactions between tumor cells and matrix proteins are a key determinant in the tumor progression and metastases dissemination, which represent the main uncontrolled problem in cancer therapeutics. 2 Among these ECM proteins, type I collagen represents the major component in the body connective tissues through which tumor cells usually migrate to form metastases, and which can be used as a preintravasation microenvironment. 3 In fact, specific sites of type I collagen are recognized by integrins to promote cell attachment to the matrix fibrillar network, 4 enabling the trigger migration cycle of cells.
Due to its particular longevity, this key matrix protein is a preferential target for posttranslational modifications during chronological aging; these age-related modifications are cumulative and irreversible 5 and act on the physical and biomechanical properties of collagen networks. 6 The main mechanism is the accumulation of the so-called advanced glycation endproducts (AGEs), which are cross-linking compounds resulting from the well-known nonenzymatic glycation process. 7 As a result, AGEs are responsible for changes in the structure and function of collagen networks, which detrimentally affect cancer cell/ matrix interactions 8 and are suggested to be associated with cancer development.
In this work, we have analyzed HT1080 human fibrosarcoma cells, known to be highly invasive, grown on two-dimensional coatings of different-age type I collagens. This coating model has previously been shown as a pertinent cell culture system for studying the proliferative capacity and migratory parameters of tumor cells at the single-cell level. 9 Cell/matrix interactions are most often studied by conventional techniques of cellular and molecular biology or biochemistry such as studying regulation of proteases secretion 10 or focal contacts formation. 11 Other approaches based on the dynamic study of cell/matrix contacts use fluorescence lifetime microscopy techniques. However, these require labeling cells with specific fluorophores. 12 In this study, we investigated the tumor cells grown on different-age collagen networks by synchrotron-based Fourier transform infrared (S-FTIR) microspectroscopy with the objective to gain insights into the cell/matrix interactions regions. The different-age collagen matrix models are based on the extraction of native and fibrillar type I collagens from newborn, youngadult, and old-adult rats. These models allow the analysis of the impact of aging on the cancer cell/collagen interactions.
Using S-FTIR microspectroscopy permits a label-free, direct, and rapid analysis of biological samples with a high spatial resolution. Furthermore, the IR radiation is not damaging for cells and thus allows studying them while preserving their integrity. 13 Vibrational microspectroscopy has already proven its ability to analyze tumor cells at the single-cell level. Raman spectral imaging has been used for extracting spectral signals from nucleus and cytoplasm with their biochemical attribution, showing the distribution of cell components at the subcellular scale. 14 In the same way, the S-FTIR microspectroscopy has been shown to be able to differentiate cancer cells from their normal counterparts, showing the potential of FTIR methodologies in cancer clinical diagnosis. 15 A recent study by both Raman and FTIR spectroscopies evaluated the effect of substrate coating on molecular and functional changes in adherent cells using an analysis of point spectra. 16 Here, we chose to approach the single-cell level analysis using a synchrotron source to benefit from its high intrinsic brightness and its ability to provide high-quality spectra at diffraction-limited spot sizes. Although it is possible to obtain reasonable cell spectra with a conventional Globar® source, in this study the use of the IR synchrotron source and small apertures is justified because our aim was to attempt to identify infrared spectral features from specific cell/collagen interaction regions. Thus, with this set-up, good signal/noise data could be reached. A spectral data processing via clustering methods was used to analyze the spectral maps, to gain insight into the spectral features of cell/matrix interaction regions and invasion fronts during the tumor progression process and to evaluate the effect of aged collagen networks on these spectral features.

Cell Line
The HT1080 human fibrosarcoma cell line (CCL-121) was purchased from the American Type Culture Collection (ATCC, Rockville, Maryland) and cultured in minimum essential media (MEM) with Earle salts and Glutamax (Invitrogen, Cergy-Pontoise, France) supplemented with 10% fetal bovine serum (Invitrogen) and 1% penicillin-streptomycin (Invitrogen). Cultures were maintained at 37°C in a humidified atmosphere containing 5% CO 2 (v/v), and cells were routinely passaged at preconfluency using 0.05% trypsin-0.53-mM ethylenediamine tetraacetate (EDTA) (Invitrogen) and screened for the presence of mycoplasma using polymerase chain reaction (PCR) methods.

Preparation of Different-Age Type I Collagens
Native type I collagens were extracted from tail tendons of different age Sprague-Dawley rats, newborns (a few days), 2 months (young adults), and 2 years (old adults), following the protocol previously described. 17 Briefly, acid-soluble type I collagens were obtained by 0.5-M acetic acid extraction then purified by dialysis against distilled water and freeze-dried. This preparation without pepsinization provides collagens with intact telopeptides, in contrast with most commercially available type I collagens. The fibrillar, purified, and native type I collagens of different ages were then stored at −80°C until further use. As the collagen extracted from young-adult animals is commonly used in the field of biology to construct culture models, it will be considered as the control sample of our different-age collagen range.

Cell Culture on Different-Age Type I Collagen Coatings
To prepare collagen coatings, type I collagens of each age were solubilised in 0.018-M acetic acid and deposited on calcium fluoride windows (25 mm × 1 mm) (Crystran, Dorset, United Kingdom) according to a concentration of 50 μg of protein per cm 2 . Coated surfaces were dried overnight at room temperature under sterile conditions and rinsed with distilled water before cell seeding. Then, CaF 2 windows were placed in a 6well plate and HT1080 cells were seeded at a concentration of 5000 cell∕ml (3 ml of cell suspension per well) and cells were grown for 48 h. Samples were then fixed with 4% paraformaldehyde in phosphate buffer for 15 min and rinsed with distilled water. For each condition of collagen age, three different-coated samples were prepared and seeded with HT1080 cells, and then between 10 and 15 individual cells for each collagen age conditions were mapped by S-FTIR microspectroscopy.

Synchrotron-FTIR Microspectroscopy
S-FTIR spectral acquisitions were performed with the synchrotron source of the B22-multimode infrared imaging and microspectroscopy (MIRIAM) beamline at the DIAMOND Light Source synchrotron facilities (Didcot, UK), using a Vertex 80v FT-IR spectrometer coupled to a HYPERION 3000 FT-IR microscope (Bruker Optics, Coventry, United Kingdom) controlled by the Opus 6.5 software. The S-FTIR data were directly collected on CaF 2 windows in the transmission mode with a 36× Cassegrain objective using a 10 × 10-μm 2 aperture (step size of 10 μm for whole cells and 3 μm for part of the cell including cell/collagen contact areas). Each spectrum was a coaddition of 128 scans at 4 cm −1 resolution recorded between 4000 and 1000 cm −1 . During the acquisition, the system was operating under vacuum and prior to each spectral acquisition, a background spectrum was recorded at 256 scans accumulations on a blank part of the same CaF 2 window. Each individual sample spectrum was rationed to the background spectrum to reduce any atmospheric effects. Cells grown on collagen coatings were individually mapped by selecting a region of about 80 × 90 μm 2 . This allowed the analysis of the entire single cell and its surrounding collagen substrate. As a function of the cell size (average size of 40 × 20 μm 2 ) and of the acquisition parameters, the time for data collection from one cell to another varied from 60 to 180 min. Each cell was individually mapped.

Data Preprocessing
The S-FTIR spectral data were preprocessed by an automated method based on the classical extended multiplicative signal correction (EMSC) algorithm implemented in the MATLAB 7.2 software (The Mathworks, Natick, Massachusetts, USA). Briefly, EMSC allows correcting the spectra for the physical light scattering effects that are different from the chemical light absorbance effects in order to retain only the spectral variability of the sample. 18,19 This correction algorithm is able to remove outlier spectra or spectra with a low signal-to-noise (S/N) ratio, and it also allows performing a spectral baseline correction and vector-normalization in order to render them comparable.

Spectral Clustering Methods
Spectral data analyses and clusterings were performed using home-made algorithms written in the MATLAB 7.2 software. S-FTIR data, recorded on areas showing few cells on different-age collagen coatings, were compared using three different unsupervised clustering methods applied on the 1800 to 1000 cm −1 spectral range: hierarchical clustering analysis (HCA), k-means classification, and fuzzy C-means (FCM) clustering. HCA allows regrouping spectra on a minimal distance criterion using Euclidean distances and Ward's algorithm, and spectra with a high degree of homogeneity are grouped together. 20,21 K-means clustering consists of a nonhierarchical clustering technique partitioning spectra into preassigned number of clusters by randomly selecting cluster centers. 22,23 The FCM clustering is based on the minimization of the sum of weighted distance measures between each spectrum (pixel) and each centroid, allowing one to assign each spectrum to every cluster with an associated membership value comprised between 0 and 1. 24 The FCM images were constructed with redundancy-based algorithm (RBA)-optimized parameters (number of clusters K and fuzziness index m). This model, previously developed for tissue spectral classification, 25 allows providing a biologically relevant clustering. For both k-means and FCM classifications of single-cell spectral maps, the calculation was performed on at least 12 different cells for each collagenage condition, applying the same clustering parameters (number of clusters and pseudocolor coding).

Comparison of Conventional FTIR and S-FTIR Spectra at the Single-Cell Level
A synchrotron source was used to analyze HT1080 cells grown on coating substrates. Figure 1 displays the mean FTIR spectra from HT1080 cells acquired with a conventional Globar ® source (in gray) and a synchrotron source (in dark), with the same aperture size of 10 × 10 μm 2 at the sample. This aperture size was necessary to obtain single-cell spectra, and it appeared clear that at this beam size it is important to use a synchrotron source due to the better S/N ratio obtained with the highly collimated and brilliant source. Further, the S-FTIR spectrum exhibited the vibrational modes of lipids (3100 to 2800 cm −1 ) and proteins (1700 to 1500 cm −1 ) commonly investigated in biological samples with a higher intensity. Using a synchrotron source enabled the collection of spatially resolved chemical and informative molecular information from a single cell, as previously established. 26 HT1080 tumor cells in contact with their surrounding collagen substrate were individually mapped, and at least 12 different individual cells per collagen age condition were analyzed. These conditions were sufficient to highlight the weak spectral differences observed in a reproducible way and were consistent between cells.

K -means Classification of S-FTIR Maps from HT1080 Tumor Cells Grown on Different-Age Collagen Coatings
HT1080 tumor cells were grown on different-age type I collagen substrates used here as a model of increasing-age ECM proteins. S-FTIR spectral maps recorded on these samples were first analyzed by a common k-means clustering using 4 clusters in order to consider the three-different collagen age conditions and the cellular component. K-means clustering data presented in Fig. 2 are representative of results obtained for three individual cells for each age condition. It must be noted here that the three images were processed together with the k-means clustering procedure so as to obtain matching colors when the chemical  morphology with an elongated cell body. K-means clustering revealed that for each age condition, cellular components were grouped into a single cluster (cluster 3, in blue), while the three coating conditions were assigned to three independent clusters as a function of collagen age, i.e., cluster 4 (in purple) for the newborn collagen substrate, cluster 2 (in green) for the young-adult condition (control), and cluster 4 (in yellow) for the old-adult coating. The dendrogram of the cluster centroids with the corresponding spectra displayed in Fig. 2(d) shows that the cluster attributed to the newborn collagen substrate is well separated from the two other collagen ages and from the cellular component. K-means clustering allows a good discrimination of coatings as a function of collagen age and a reproducible assignment of the HT1080 cell spectral fingerprint was achieved. However, one limitation of k-means clustering is that it provides a too uniform representation of collagen substrates. after RBA-estimation of the optimal FCM parameters (K ¼ 4 and m ¼ 1.83) going from the intracellular compartments to the extracellular microenvironment with cluster 1 assigned to cell nuclear components, cluster 2 to cytoplasmic components, cluster 3 to cell/collagen contact areas, and cluster 4 to intact collagen. The centroid spectra corresponding to these clusters as well as HCA classification are shown in Figs. 3(c) and 3(d), respectively. The dendrogram exhibits a clear discrimination between the four clusters. Similar results were obtained with the newborn and young-adult collagen coatings (data not shown). Following these observations, we analyzed spectra originating from the intact collagen areas (cluster 4) and those from the cell/collagen interaction areas (cluster 3). Spectra were extracted after FCM analysis of the spectral maps from three individual cells for each collagen-age condition.

Analysis of different-age type I intact collagen coatings
In order to highlight the spectral changes in the infrared signal of collagen as a function of age, spectra corresponding to cluster 4 that we define as intact collagen (away from the cell/collagen contact areas) were extracted from the FCM images for each age condition, and compared in the 1800 to 1000 cm −1 spectral range [ Fig. 4(a)]. The Amide I band profile with a maximum absorbance at 1653 cm −1 did not exhibit any significant changes as a function of the collagen age. An absence of modifications in the Amide III band (1300 to 1150 cm −1 ) can also be noticed.
Concerning the Amide II band, an upshift was observed between newborn (1547 cm −1 ) and, young and old adult (1552 cm −1 ) collagens. Furthermore, a band profile modification in the sugar region (1150 to 1000 cm −1 ), generally related to the presence of AGEs, can be observed between newborns and adults. Taken together, these spectral modifications, although subtle, could be exploited by HCA to analyze spectra from differentaged type I collagens [ Fig. 4(b)]. The generated dendrogram showed a good discrimination with age since all spectra were correctly assigned to their respective age groups. The group corresponding to the newborns is the furthest from the two other age conditions. Furthermore, by inspecting the heterogeneity scale, the old-adult group appeared to be more homogeneous compared with the newborn and young-adult groups.

Analysis of cell/collagen interaction areas
This was performed by considering spectra extracted from the FCM images (cluster 3) for each collagen-age condition. Figure 5(a) depicts the mean spectra corresponding to the interaction areas between HT1080 cells and the three-different age collagens. For all conditions, the Amide I band was at 1654 cm −1 , whereas the Amide II band was observed at 1551 cm −1 for newborns and young adults, and at 1553 cm −1 for old adults. No significant shifts were observed for the Amide III band (1300 to 1150 cm −1 ) and the sugar region (1150 to 1000 cm −1 ) upon comparing the three age conditions. However, the dendrogram obtained by HCA clustering [ Fig. 5(b)] showed discrimination between the three conditions of cell/collagen contact areas. This may be explained by changes in relative intensities of bands, for instance the Amide I, II, and III bands, and the lower frequencies. Fig. 4 Analysis of S-FTIR spectra from different-aged type I collagen coatings with the mean spectra presented in the 1800 to 1000 cm −1 spectral range (a). Spectra were extracted from cluster 4 of the FCM images for each collagen age group and defined as "intact collagen spectra." The difference observed for the peak maximum of the Amide II band as a function of collagen age is indicated by the arrow. Results of HCA clustering (b) are displayed via a dendrogram in which spectra are clustered according to their heterogeneity degree. Fig. 5 Analysis of S-FTIR spectra from cell/collagen peripheral areas as a function of collagen age with the mean spectra presented in the 1800 to 1000 cm −1 spectral range (a). Spectra were extracted from the cluster 3 of FCM images for each collagen age group and defined as "cell/collagen contact spectra." Results of HCA clustering (b) are displayed via a dendrogram in which spectra are clustered according to their heterogeneity degree.
In order to highlight the possible existing age-related differences in cell/collagen contact areas, we have analyzed for each condition, the difference spectra obtained by subtracting the age-corresponding spectrum of intact collagen from that of the cell/collagen contact area. Thus, the contribution of intact collagen was minimized. These difference spectra are displayed in Figs. 6(a), 6(b), and 6(c), for newborns, young and old adults, respectively, and are offset for clarity. From this analysis, it can be observed that spectral differences showed a similar profile in the protein absorption bands (Amide I, II, and III) for newborns and old adults, and in the sugar region (1150 to 1000 cm −1 ) for young and old adults.
These two data sets (clusters 3 and 4) were further analyzed by comparing two AGEs-related spectral markers that have been previously reported. These spectral markers are shown in Fig. 7, where cluster centroid spectra corresponding to different-aged collagens were compared for intact collagen [ Fig. 7(a)] and for contact areas [ Fig. 7(b)]. The first marker of AGEs, I 1032 ∕I 1655 , was calculated by ratioing the intensity of the 1032 cm −1 sugar band to that of the 1655 cm −1 Amide I band. 27 The second AGEs marker, A sugar ∕A amide II , was based on the work of Birarda et al., 28 where the authors calculated the ratio of the band area of the sugar region (1180 to 1000 cm −1 ) to that of the Amide II band (1600 to 1500 cm −1 ). An increase of these two markers suggests an increase in AGEs. The results are summarized in Table 1 where it can be seen that both markers showed a similar tendency for the studied cases. The intensity ratio I 1032 ∕I 1655 and the band area ratio A sugar ∕A amide II both increased when the old adult data were compared to those of the newborn and the young adult in the case of intact collagens. In contrast with intact collagens, the two ratios calculated for the contact areas remained constant for the three age groups and exhibited lower values; the latter being more marked (twofold to threefold) for old adults.

Discussion
Understanding the interactions between cancer cells and their microenvironment constitutes an important step in experimental oncology to gain better insights into key cellular processes, such as proliferation, differentiation, and invasion. Type I collagen, a major component of the tumor microenvironment, is an ideal candidate for investigating these interactions while taking into account the process of protein aging. In this study, we employed the S-FTIR microspectroscopy to acquire spatially resolved spectral biomolecular information in a model of single human HT1080 cancer cells interacting with different-aged type I collagen networks. Even if infrared analysis at the cellular level has already been performed with conventional Globar sources, our aim was to acquire high spatially resolved chemical information not only from the single cell itself but also from the cell/collagen contact regions and from the intact collagen. This was feasible with small apertures by combining FTIR microspectroscopy with the high brightness of the synchrotron source. We also expected the spectral differences to be weak, but with Fig. 6 Difference spectra calculated by subtracting each corresponding intact collagen spectrum from that of the cell/collagen contact spectrum. This was computed for each collagen age condition: newborn (top), young adult (middle), and old adult (bottom). Spectra were offset for clarity and the regions with higher changes are highlighted by the gray bars.
this set-up good quality and exploitable spectral maps could be achieved. The FTIR microspectroscopy of single isolated cells has often been prone to physical effects like the Mie and resonant Mie scattering and dispersive phenomena, causing spectral artifacts that can lead to spectral misinterpretation. These were previously described for single cells or small samples directly analyzed on infrared substrates. [29][30][31] In our data, these effects were weak or nonexistent because although we analyzed single cells, they were not completely isolated but were surrounded by the collagen matrix. Therefore, our S-FTIR spectra were preprocessed using the conventional EMSC algorithm 18 that did not take these artifacts into account.
The high brightness of the S-FTIR approach has previously been used to analyze the proteolytic activity of tumor cells in gelatin matrices. 32 However, this is the first study that focuses on the collagen aging factor in the cell/ECM model. The cell's surrounding microenvironment is indeed an essential criterion that needs to be taken into account for studying tumor processes, and previous works have demonstrated that posttranslational modifications of type I collagen occurring during aging or age-related pathological processes have an impact on the behavior of HT1080 cancer cells. 9 The three age groups studied here correspond respectively to physiological states of newborns, young adults, and old adults, 33 thus allowing us to work on a model representative of a pertinent aging range.
Color-coded spectral images of single cells grown onto different-aged collagen coatings were reconstructed using k-means and FCM clustering methods. The first method allowed the discrimination of the matrix information from the cellular entity. However, one limitation of the k-means is that each pixel is assigned to only one cluster with the consequence of a lack of nuance to distinguish between intact collagen and collagen interacting with the cell. This drawback was overcome by using FCM, which allowed each pixel to be assigned to every cluster with an associated membership value varying between 0 (no class membership) and 1 (highest degree of cluster membership). 25 Hence, this approach permitted us to obtain a nuance in the cluster images, revealing a progressive variation in the biochemical information upon going from the intracellular region (clusters 1 and 2), to the cell periphery (cluster 3), and to the intact collagen network (cluster 4).
The analysis of intact collagen showed that Amide I and III bands did not exhibit any significant shift as a function of age, indicating that the triple helical structure of the protein was not affected during aging. On the other hand, the upshift observed in the Amide II band (when comparing newborn with young and old adults) suggests weak conformational changes in the tertiary structure of collagen, such as turns of amino-acid exposure, due to an accumulation of cross-linking AGEs with age. We previously reported such spectral differences for in vitro glycated collagens. 27 We hypothesize that, with the accumulation of cross-linking compounds such as AGEs, the backbone and the rigidity of the protein increased, leading to changes in the amino-acid exposure. The old adult age-group also exhibited a very distinct cluster in the HCA analysis. This could be explained by the age-related modifications in biomechanical properties due to the AGEs addition, leading to an increase of nonenzymatic cross-links. 6,8 Furthermore, the increase in AGEs with age can be monitored via the increase of two spectral indicators, the intensity ratio (I 1032 ∕I 1655 ) 27 and the band area ratio (A sugar ∕A amide II ). 28 The high ratios obtained in old adults compared with younger groups correlate well with our recently Fig. 7 Analysis of cluster centroid spectra from intact collagens (a) and from cell/collagen contact areas (b) as a function of collagen-age. The two filled spectral bands (sugar band between 1180 and 1000 cm −1 , and the Amide II band between 1600 and 1500 cm −1 were then used for calculating ratios previously described as advanced glycation endproducts (AGEs)-related spectral markers (see Table 1). Table 1 Advanced glycation end products (AGEs)-related spectral markers as a function of collagen age. The intensity ratio I 1032 ∕I 1655 and the band area ratio A sugar ∕A amide II were calculated for both intact collagen and cell/collagen contact data sets, and compared as a function of collagen-age. reported auto-fluorescence and electrophoresis data that support an increase of AGEs with age. 34 Another previous study confirmed that modifications in the infrared band of sugars (1150 to 1000 cm −1 ), especially the increase of the carbohydrate peak intensity, are directly and proportionally linked to the glycation level. 35 All these observations clearly highlight that the sugar band constitutes a good and semi-quantitative infrared marker of the AGEs accumulation occurring during aging and age-related processes. The clustering results showed that the highest spectral distances were observed for the newborn collagen I group; this could be explained by the natural history of collagen from gestation to childhood periods during which collagen fibers are not yet mature and present a defect of their alignment in spite of a high content in type I collagen. 36,37 The use of the S-FTIR label-free biophotonic method constitutes an approach complementary to the conventional biochemical techniques for investigating the impact of ECM proteins on the tumor cell behavior. Here, we focused on the peripheral contact zones between HT1080 cancer cells and type I collagen networks.
Our data show that the calculation of the two AGEs indicators described above was leveled to constant values for the contact zones of the three age-groups. In addition, in the case of the old adult contact zone, a twofold to threefold drastic reduction of these indicators was observed compared with the intact collagen age-groups, which tends to suggest a masking of the biochemical spectral information. At the level of the protein structure, from the difference spectra, it appears that the old adult and newborn collagen contact zones exhibit a quite similar profile in the corresponding bands, whereas for the young adult, a perturbation of its secondary structure is noticed as witnessed by the changes observed at the level of the Amide I, II, and III bands. These findings are interesting and to the best of our knowledge have not been previously reported. The mechanisms of interaction at the level of these contact zones and as a function of age appear quite complex and require other complementary investigations for understanding them. Methods like Raman microspectroscopy could produce complementary vibrational information and other biophotonic approaches like polarized second harmonic generation could help to image the cell/collagen contact zones and inform on morphological parameters, such as the molecular order, the degree of orientational freedom, the molecular constraint, and rigidity of the collagen fibers. 38 Indeed, these should be supported by conventional biochemical analysis.

Conclusion
This study demonstrates the potential of synchrotron-based infrared microspectroscopy to map single-tumor cells grown on a matrix substrate, in a direct, label-free, and noninvasive way. For the different collagen age-groups, it helped to provide specific information on the cell/collagen peripheral contact zones, where key processes take place during tumor progression. The application of image clustering techniques allowed differentiating the spectral information of the collagen coatings from that of the cell and further providing a gradation in the cluster images that revealed a progressive variation in the biochemical information upon going from the intracellular region, to the cell periphery, and to the intact collagen network. Two indicators based on sugar/protein absorption bands either via the ratio of band intensities or via that of band areas showed a net increase of AGEs accumulation in the old-adult intact collagens. This tendency disappeared in the presence of the HT1080 cells due to a masking of the biochemical information. Such S-FTIR data are reported for the first time and necessitate complementary biochemical and morphological investigations to achieve a better insight into the modifications that take place in the contact zones of the different-aged collagens. This approach holds promises for single-live cell studies with FTIR microspectroscopy and, at the same time, minimizes undesired optical effects like scattering and dispersion artifacts. This feasibility study, therefore, opens the way for further investigations on FTIR imaging of single-tumor cells while at the same time integrating the age-related modifications of ECM components. It appears to be promising to apply this approach to study single cells growing on more complex culture models such as three-dimensional matrices which can better mimic the cell microenvironment. 39