Dynamic fluorescence lifetime imaging based on acousto-optic deflectors

Wei Yan; Xiao Peng; Jing Qi; Jian Gao; Shunping Fan; Qi Wang; Junle Qu; Hanben Niu

doi:10.1117/1.JBO.19.11.116004

6 November 2014 Dynamic fluorescence lifetime imaging based on acousto-optic deflectors

Wei Yan; Xiao Peng; Jing Qi; Jian Gao; Shunping Fan; Qi Wang; Junle Qu; Hanben Niu

Author Affiliations +

J. of Biomedical Optics, 19(11), 116004 (2014). https://doi.org/10.1117/1.JBO.19.11.116004

Abstract

We report a dynamic fluorescence lifetime imaging (D-FLIM) system that is based on a pair of acousto-optic deflectors for the random regions of interest (ROI) study in the sample. The two-dimensional acousto-optic deflector devices are used to rapidly scan the femtosecond excitation laser beam across the sample, providing specific random access to the ROI. Our experimental results using standard fluorescent dyes in live cancer cells demonstrate that the D-FLIM system can dynamically monitor the changing process of the microenvironment in the ROI in live biological samples.

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Wei Yan, Wei Yan, Xiao Peng, Xiao Peng, Jing Qi, Jing Qi, Jian Gao, Jian Gao, Shunping Fan, Shunping Fan, Qi Wang, Qi Wang, Junle Qu, Junle Qu, Hanben Niu, Hanben Niu, } "Dynamic fluorescence lifetime imaging based on acousto-optic deflectors," Journal of Biomedical Optics 19(11), 116004 (6 November 2014). https://doi.org/10.1117/1.JBO.19.11.116004 . Submission:

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