11 November 2014 <italic<In vivo</italic< label-free quantification of liver microcirculation using dual-modality microscopy
Author Affiliations +
J. of Biomedical Optics, 19(11), 116006 (2014). doi:10.1117/1.JBO.19.11.116006
Abstract
Microcirculation lesion is a common symptom of chronic liver diseases in the form of vasculature deformation and circulation alteration. In acute to chronic liver diseases such as biliary atresia, microcirculation lesion can have an early onset. Detection of microcirculation lesion is meaningful for studying the progression of liver disease. We have combined wide-field fluorescence microscopy and a laser speckle contrast technique to characterize hepatic microcirculation <italic<in vivo</italic< without labeling in a bile-duct ligation rat fibrosis model of biliary atresia. Through quantitative image analysis of four microcirculation parameters, we observed significant microcirculation lesion in the early to middle stages of fibrosis. This bimodal imaging method is useful to assess hepatic microcirculation lesion for the study of liver diseases.
Jie Yan, Yuzhan Kang, Shuoyu Xu, Lee-Ling S. Ong, Shuangmu Zhuo, Ralph M. Bunte, Nanguang Chen, H. Harry Asada, Peter T. C. So, Ian R. Wanless, Hanry Yu, "<italic<In vivo</italic< label-free quantification of liver microcirculation using dual-modality microscopy," Journal of Biomedical Optics 19(11), 116006 (11 November 2014). http://dx.doi.org/10.1117/1.JBO.19.11.116006
JOURNAL ARTICLE
6 PAGES


SHARE
KEYWORDS
Liver

In vivo imaging

Microscopy

Speckle

Luminescence

Laser speckle contrast imaging

Tissues

Back to Top