19 November 2014 Photoacoustic lymphatic imaging with high spatial-temporal resolution
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Abstract
Despite its critical function in coordinating the egress of inflammatory and immune cells out of tissues and maintaining fluid balance, the causative role of lymphatic network dysfunction in pathological settings is still understudied. Engineered-animal models and better noninvasive high spatial-temporal resolution imaging techniques in both preclinical and clinical studies will help to improve our understanding of different lymphatic-related pathologic disorders. Our aim was to take advantage of our newly optimized noninvasive wide-field fast-scanning photoacoustic (PA) microcopy system to coordinately image the lymphatic vasculature and its flow dynamics, while maintaining high resolution and detection sensitivity. Here, by combining the optical-resolution PA microscopy with a fast-scanning water-immersible microelectromechanical system scanning mirror, we have imaged the lymph dynamics over a large field-of-view, with high spatial resolution and advanced detection sensitivity. Depending on the application, lymphatic vessels (LV) were spectrally or temporally differentiated from blood vessels. Validation experiments were performed on phantoms and
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Catherine Martel, Catherine Martel, Junjie Yao, Junjie Yao, Jun Zou, Jun Zou, Chih-Hsien Huang, Chih-Hsien Huang, Gwendalyn J. Randolph, Gwendalyn J. Randolph, Lihong V. Wang, Lihong V. Wang, } "Photoacoustic lymphatic imaging with high spatial-temporal resolution," Journal of Biomedical Optics 19(11), 116009 (19 November 2014). https://doi.org/10.1117/1.JBO.19.11.116009 . Submission:
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