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21 November 2014 Multiphoton gradient index endoscopy for evaluation of diseased human prostatic tissue ex vivo
David M. Huland, Manu Jain, Dimitre G. Ouzounov, Brian D. Robinson, Diana S. Harya, Maria M. Shevchuk, Paras Singhal, Chris Xu, Ashutosh K. Tewari
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Journal of Biomedical Optics, Vol. 19, Issue 11, 116011 (November 2014). https://doi.org/10.1117/1.JBO.19.11.116011
Abstract
Multiphoton microscopy can instantly visualize cellular details in unstained tissues. Multiphoton probes with clinical potential have been developed. This study evaluates the suitability of multiphoton gradient index (GRIN) endoscopy as a diagnostic tool for prostatic tissue. A portable and compact multiphoton endoscope based on a 1-mm diameter, 8-cm length GRIN lens system probe was used. Fresh ex vivo samples were obtained from 14 radical prostatectomy patients and benign and malignant areas were imaged and correlated with subsequent H&E sections. Multiphoton GRIN endoscopy images of unfixed and unprocessed prostate tissue at a subcellular resolution are presented. We note several differences and identifying features of benign versus low-grade versus high-grade tumors and are able to identify periprostatic tissues such as adipocytes, periprostatic nerves, and blood vessels. Multiphoton GRIN endoscopy can be used to identify both benign and malignant lesions in ex vivo human prostate tissue and may be a valuable diagnostic tool for real-time visualization of suspicious areas of the prostate.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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David M. Huland, Manu Jain, Dimitre G. Ouzounov, Brian D. Robinson, Diana S. Harya, Maria M. Shevchuk, Paras Singhal, Chris Xu, and Ashutosh K. Tewari "Multiphoton gradient index endoscopy for evaluation of diseased human prostatic tissue ex vivo," Journal of Biomedical Optics 19(11), 116011 (21 November 2014). https://doi.org/10.1117/1.JBO.19.11.116011
Published: 21 November 2014
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Cited by 17 scholarly publications.
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KEYWORDS
Tissues
Endoscopy
GRIN lenses
Prostate
Endoscopes
Tissue optics
Tumors
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