18 December 2014 In-fiber photo-immobilization of a bioactive surface
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We demonstrate the first in-fiber light-induced bioactive biotin-functionalization via photobleaching fluorophore-conjugated biotin. Photobleaching the fluorophores generated free radicals that bind to the albumin-passivated inner surface of pure silica photonic crystal fiber. The subsequent attachment of dye-conjugated streptavidin to the bound biotin qualified the photo-immobilization process and demonstrated a potential for the construction of in-fiber macromolecular assemblies or multiplexes. Compared with other in-fiber bioactive coating methods, the proposed light-induced technique requires only a low-power light source, without the need for additional preactivation steps or toxic chemical reagents. This method, hence, enables a simple and compact implementation for potential biomedical applications.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Elizabeth Lee, Elizabeth Lee, Derrick Yong, Derrick Yong, Xia Yu, Xia Yu, Hao Li, Hao Li, Chi Chiu Chan, Chi Chiu Chan, } "In-fiber photo-immobilization of a bioactive surface," Journal of Biomedical Optics 19(12), 120502 (18 December 2014). https://doi.org/10.1117/1.JBO.19.12.120502 . Submission:

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