24 December 2014 Combined lineage mapping and gene expression profiling of embryonic brain patterning using ultrashort pulse microscopy and image registration
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Abstract
During embryogenesis, presumptive brain compartments are patterned by dynamic networks of gene expression. The spatiotemporal dynamics of these networks, however, have not been characterized with sufficient resolution for us to understand the regulatory logic resulting in morphogenetic cellular behaviors that give the brain its shape. We have developed a new, integrated approach using ultrashort pulse microscopy [a high-resolution, two-photon fluorescence (2PF)-optical coherence microscopy (OCM) platform using 10-fs pulses] and image registration to study brain patterning and morphogenesis in zebrafish embryos. As a demonstration, we used time-lapse 2PF to capture midbrain-hindbrain boundary morphogenesis and a
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Holly C. Gibbs, Colin R. Dodson, Yuqiang Bai, Arne C. Lekven, Alvin T. Yeh, "Combined lineage mapping and gene expression profiling of embryonic brain patterning using ultrashort pulse microscopy and image registration," Journal of Biomedical Optics 19(12), 126016 (24 December 2014). https://doi.org/10.1117/1.JBO.19.12.126016 . Submission:
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