Mueller matrix three-dimensional directional imaging of collagen fibers

Pål G. Ellingsen; Lars Martin S. Aas; Vegard S. Hagen; Rajesh Kumar; Magnus B. Lilledahl; Morten Kildemo

doi:10.1117/1.JBO.19.2.026002

6 February 2014 Mueller matrix three-dimensional directional imaging of collagen fibers

Pål G. Ellingsen, Lars Martin S. Aas, Vegard S. Hagen, Rajesh Kumar, Magnus B. Lilledahl, Morten Kildemo

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Journal of Biomedical Optics, Vol. 19, Issue 2, 026002 (February 2014). https://doi.org/10.1117/1.JBO.19.2.026002

Abstract

A method for measuring three-dimensional (3-D) direction images of collagen fibers in biological tissue is presented. Images of the 3-D directions are derived from the measured transmission Mueller matrix images (MMIs), acquired at different incidence angles, by taking advantage of the form birefringence of the collagen fibers. The MMIs are decomposed using the recently developed differential decomposition, which is more suited to biological tissue samples than the common polar decomposition method. Validation of the 3-D direction images was performed by comparing them with images from second-harmonic generation microscopy. The comparison found a good agreement between the two methods. It is envisaged that 3-D directional imaging could become a useful tool for understanding the collagen framework for fibers smaller than the diffraction limit.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Pål G. Ellingsen, Lars Martin S. Aas, Vegard S. Hagen, Rajesh Kumar, Magnus B. Lilledahl, and Morten Kildemo "Mueller matrix three-dimensional directional imaging of collagen fibers," Journal of Biomedical Optics 19(2), 026002 (6 February 2014). https://doi.org/10.1117/1.JBO.19.2.026002

Published: 6 February 2014

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