10 March 2014 Ultrasensitive opto-microfluidic immunosensor integrating gold nanoparticle–enhanced chemiluminescence and highly stable organic photodetector
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Abstract
The expensive fabrication of current opto-microfluidic sensors is a barrier to the successful adoption of these devices in point-of-care testing. This work reports a simple inexpensive opto-microfluidic device incorporating a poly(dimethylsiloxane)-glass hybrid microfluidic chip modified with gold nanoparticles and a high-detectivity, high-stability organic photodetector. The enhancing effect of the gold nanoparticles on horseradish peroxidase-luminol-H 2 O 2 chemiluminescence was exploited in rapid single-analyte immunoassays. The limit of detection for 17-β estradiol was 2.5  pg/ml , which is ∼200 times more sensitive than previously reported chemiluminescent immunosensors employing other organic photodetectors. Detection was also demonstrated in complex media, including natural water and blood serum.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Nuno Miguel Matos Pires, Tao Dong, "Ultrasensitive opto-microfluidic immunosensor integrating gold nanoparticle–enhanced chemiluminescence and highly stable organic photodetector," Journal of Biomedical Optics 19(3), 030504 (10 March 2014). https://doi.org/10.1117/1.JBO.19.3.030504 . Submission:
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