6 March 2014 Dependence of optical scattering from Intralipid in gelatin-gel based tissue-mimicking phantoms on mixing temperature and time
Author Affiliations +
Abstract
Intralipid is widely used as an optical scattering agent in tissue-mimicking phantoms. Accurate control when using Intralipid is critical to match the optical diffusivity of phantoms to the prescribed value. Currently, most protocols of Intralipid-based hydrogel phantom fabrication focus on factors such as Intralipid brand and concentration. In this note, for the first time to our knowledge, we explore the dependence of the optical reduced scattering coefficient (at 532 nm optical wavelength) on the temperature and the time of mixing Intralipid with gelatin-water solution. The studied samples contained 1% Intralipid and were measured with oblique-incidence reflectometry. It was found that the reduced scattering coefficient increased when the Intralipid-gelatin-water mixture began to solidify at room temperature. For phantoms that had already solidified completely, the diffusivity was shown to be significantly influenced by the temperature and the duration of the mixing course. The dependence of the measured diffusivity on the mixing conditions was confirmed by experimental observations. Moreover, the mechanism behind the dependence behavior is discussed.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Puxiang Lai, Puxiang Lai, Xiao Xu, Xiao Xu, Lihong V. Wang, Lihong V. Wang, } "Dependence of optical scattering from Intralipid in gelatin-gel based tissue-mimicking phantoms on mixing temperature and time," Journal of Biomedical Optics 19(3), 035002 (6 March 2014). https://doi.org/10.1117/1.JBO.19.3.035002 . Submission:
JOURNAL ARTICLE
7 PAGES


SHARE
RELATED CONTENT

Light-scattering model for biological tissue
Proceedings of SPIE (June 12 2000)
Mechanisms of optical clearing in cellular tissue
Proceedings of SPIE (April 14 2005)
Optical characterization of muscle
Proceedings of SPIE (February 28 2012)
Angular scattering functions of algae and silt an analysis...
Proceedings of SPIE (February 05 1997)

Back to Top