3 March 2014 Integrated photoacoustic, confocal, and two-photon microscope
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Abstract
The invention of green fluorescent protein and other molecular fluorescent probes has promoted applications of confocal and two-photon fluorescence microscopy in biology and medicine. However, exogenous fluorescence contrast agents may affect cellular structure and function, and fluorescence microscopy cannot image nonfluorescent chromophores. We overcome this limitation by integrating optical-resolution photoacoustic microscopy into a modern Olympus IX81 confocal, two-photon, fluorescence microscope setup to provide complementary, label-free, optical absorption contrast. Automatically coregistered images can be generated from the same sample. Imaging applications in ophthalmology, developmental biology, and plant science are demonstrated. For the first time, in a familiar microscopic fluorescence imaging setting, this trimodality microscope provides a platform for future biological and medical discoveries.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Bin Rao, Bin Rao, Florentina Soto, Florentina Soto, Daniel Kerschensteiner, Daniel Kerschensteiner, Lihong V. Wang, Lihong V. Wang, } "Integrated photoacoustic, confocal, and two-photon microscope," Journal of Biomedical Optics 19(3), 036002 (3 March 2014). https://doi.org/10.1117/1.JBO.19.3.036002 . Submission:
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