21 March 2014 High-speed multispectral confocal biomedical imaging
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J. of Biomedical Optics, 19(3), 036016 (2014). doi:10.1117/1.JBO.19.3.036016
Abstract
A new approach for generating high-speed multispectral confocal images has been developed. The central concept is that spectra can be acquired for each pixel in a confocal spatial scan by using a fast spectrometer based on optical fiber delay lines. This approach merges fast spectroscopy with standard spatial scanning to create datacubes in real time. The spectrometer is based on a serial array of reflecting spectral elements, delay lines between these elements, and a single element detector. The spatial, spectral, and temporal resolution of the instrument is described and illustrated by multispectral images of laser-induced autofluorescence in biological tissues.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Gary E. Carver, Sarah A. Locknar, William A. Morrison, V. Krishnan Ramanujan, Daniel L. Farkas, "High-speed multispectral confocal biomedical imaging," Journal of Biomedical Optics 19(3), 036016 (21 March 2014). http://dx.doi.org/10.1117/1.JBO.19.3.036016
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KEYWORDS
Confocal microscopy

Tissues

Multispectral imaging

Biomedical optics

Luminescence

Spectroscopy

Tumors

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