21 March 2014 High-speed multispectral confocal biomedical imaging
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Abstract
A new approach for generating high-speed multispectral confocal images has been developed. The central concept is that spectra can be acquired for each pixel in a confocal spatial scan by using a fast spectrometer based on optical fiber delay lines. This approach merges fast spectroscopy with standard spatial scanning to create datacubes in real time. The spectrometer is based on a serial array of reflecting spectral elements, delay lines between these elements, and a single element detector. The spatial, spectral, and temporal resolution of the instrument is described and illustrated by multispectral images of laser-induced autofluorescence in biological tissues.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Gary E. Carver, Gary E. Carver, Sarah A. Locknar, Sarah A. Locknar, William A. Morrison, William A. Morrison, V. Krishnan Ramanujan, V. Krishnan Ramanujan, Daniel L. Farkas, Daniel L. Farkas, } "High-speed multispectral confocal biomedical imaging," Journal of Biomedical Optics 19(3), 036016 (21 March 2014). https://doi.org/10.1117/1.JBO.19.3.036016 . Submission:
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