3 April 2014 Four-dimensional motility tracking of biological cells by digital holographic microscopy
Author Affiliations +
Three-dimensional profiling and tracking by digital holography microscopy (DHM) provide label-free and quantitative analysis of the characteristics and dynamic processes of objects, since DHM can record real-time data for microscale objects and produce a single hologram containing all the information about their three-dimensional structures. Here, we have utilized DHM to visualize suspended microspheres and microfibers in three dimensions, and record the four-dimensional trajectories of free-swimming cells in the absence of mechanical focus adjustment. The displacement of microfibers due to interactions with cells in three spatial dimensions has been measured as a function of time at subsecond and micrometer levels in a direct and straightforward manner. It has thus been shown that DHM is a highly efficient and versatile means for quantitative tracking and analysis of cell motility.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Xiao Yu, Jisoo Hong, Changgeng Liu, Michael Cross, Donald T. Haynie, Myung K. Kim, "Four-dimensional motility tracking of biological cells by digital holographic microscopy," Journal of Biomedical Optics 19(4), 045001 (3 April 2014). https://doi.org/10.1117/1.JBO.19.4.045001 . Submission:

Back to Top