10 April 2014 Tomographic phase microscopy of living three-dimensional cell cultures
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Abstract
A successful application of self-interference digital holographic microscopy in combination with a sample-rotation-based tomography module for three-dimensional (3-D) label-free quantitative live cell imaging with subcellular resolution is demonstrated. By means of implementation of a hollow optical fiber as the sample cuvette, the observation of living cells in different 3-D matrices is enabled. The fiber delivers a stable and accurate rotation of a cell or cell cluster, providing quantitative phase data for tomographic reconstruction of the 3-D refractive index distribution with an isotropic spatial resolution. We demonstrate that it is possible to clearly distinguish and quantitatively analyze several cells grouped in a “3-D cluster” as well as subcellular organelles like the nucleoli and local internal refractive index changes.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Arkadiusz Kuś, Arkadiusz Kuś, Michał Dudek, Michał Dudek, Björn Kemper, Björn Kemper, Małgorzata Kujawińska, Małgorzata Kujawińska, Angelika Vollmer, Angelika Vollmer, } "Tomographic phase microscopy of living three-dimensional cell cultures," Journal of Biomedical Optics 19(4), 046009 (10 April 2014). https://doi.org/10.1117/1.JBO.19.4.046009 . Submission:
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