CONFERENCE PROCEEDINGS
Advanced Search
Home > Journals > J. Biomed. Opt. > Volume 19 > Issue 4 > Article
Open Access
29 April 2014 Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy
Ana I. Doblas, Emilio Sánchez-Ortiga, Manuel Martínez-Corral, Genaro Saavedra, Jorge Garcia-Sucerquia
Author Affiliations +
Journal of Biomedical Optics, Vol. 19, Issue 4, 046022 (April 2014). https://doi.org/10.1117/1.JBO.19.4.046022
Abstract
The advantages of using a telecentric imaging system in digital holographic microscopy (DHM) to study biological specimens are highlighted. To this end, the performances of nontelecentric DHM and telecentric DHM are evaluated from the quantitative phase imaging (QPI) point of view. The evaluated stability of the microscope allows single-shot QPI in DHM by using telecentric imaging systems. Quantitative phase maps of a section of the head of the drosophila melanogaster fly and of red blood cells are obtained via single-shot DHM with no numerical postprocessing. With these maps we show that the use of telecentric DHM provides larger field of view for a given magnification and permits more accurate QPI measurements with less number of computational operations.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
Citation Download Citation
Ana I. Doblas, Emilio Sánchez-Ortiga, Manuel Martínez-Corral, Genaro Saavedra, and Jorge Garcia-Sucerquia "Accurate single-shot quantitative phase imaging of biological specimens with telecentric digital holographic microscopy," Journal of Biomedical Optics 19(4), 046022 (29 April 2014). https://doi.org/10.1117/1.JBO.19.4.046022
Published: 29 April 2014
JOURNAL ARTICLE
 8 PAGES
DOWNLOAD PAPER SAVE TO MY LIBRARY
GET CITATION
Lens.org Logo
CITATIONS
Cited by 88 scholarly publications.
Advertisement
Advertisement
KEYWORDS
Digital holography
Imaging systems
Holograms
Phase measurement
Microscopes
Phase imaging
Microscopy
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top