2 December 2013 Aptamer-conjugated gold nanorod for photothermal ablation of epidermal growth factor receptor-overexpressed epithelial cancer
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Abstract
Biomarker-specific photothermal nanoparticles that can efficiently sense markers that are overexpressed in distinguished adenocarcinomas have attracted much interest in an aspect of efficacy increase of cancer treatment. We demonstrated a promising prospect of a smart photothermal therapy agent employing anti-epidermal growth factor receptor aptamer (Apt EGFR )-conjugated polyethylene glycol (PEG) layted gold nanorods (Apt EGFR -PGNRs). The cetyltrimethylammonium bromide bilayer on GNRs was replaced with heterobifunctional PEG (COOH-PEG-SH) not only to serve as a biocompatible stabilizer and but also to conjugate Apt EGFR . Subsequently, to direct photothermal therapy agent toward epithelial cancer cells, the carboxylated PEGylated GNRs (PGNRs) were further functionalized with Apt EGFR using carbodiimide chemistry. Then, to assess the potential as biomarker-specific photothermal therapy agent of synthesized Apt EGFR -PGNRs, the optical properties, biocompatibility, colloidal stability, binding affinity, and epicellial cancer cell killing efficacy in vitro/in vivo under near-infrared laser irradiation were investigated. As a result, Apt EGFR -PGNRs exhibit excellent tumor targeting ability and feasibility of effective photothermal ablation cancer therapy.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Jihye Choi, Yeonji Park, Eun Bi Choi, Hyun-Ouk Kim, Dong Joo Kim, Yoochan Hong, Sung-Ho Ryu, Jung Hwan Lee, Jin-Suck Suh, Jaemoon Yang, Yong-Min Huh, Seungjoo Haam, "Aptamer-conjugated gold nanorod for photothermal ablation of epidermal growth factor receptor-overexpressed epithelial cancer," Journal of Biomedical Optics 19(5), 051203 (2 December 2013). https://doi.org/10.1117/1.JBO.19.5.051203 . Submission:
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