4 June 2014 Rotational multispectral fluorescence lifetime imaging and intravascular ultrasound: bimodal system for intravascular applications
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J. of Biomedical Optics, 19(6), 066004 (2014). doi:10.1117/1.JBO.19.6.066004
Abstract
We report the development and validation of a hybrid intravascular diagnostic system combining multispectral fluorescence lifetime imaging (FLIm) and intravascular ultrasound (IVUS) for cardiovascular imaging applications. A prototype FLIm system based on fluorescence pulse sampling technique providing information on artery biochemical composition was integrated with a commercial IVUS system providing information on artery morphology. A customized 3-Fr bimodal catheter combining a rotational side-view fiberoptic and a 40-MHz IVUS transducer was constructed for sequential helical scanning (rotation and pullback) of tubular structures. Validation of this bimodal approach was conducted in pig heart coronary arteries. Spatial resolution, fluorescence detection efficiency, pulse broadening effect, and lifetime measurement variability of the FLIm system were systematically evaluated. Current results show that this system is capable of temporarily resolving the fluorescence emission simultaneously in multiple spectral channels in a single pullback sequence. Accurate measurements of fluorescence decay characteristics from arterial segments can be obtained rapidly (e.g., 20 mm in 5 s), and accurate co-registration of fluorescence and ultrasound features can be achieved. The current finding demonstrates the compatibility of FLIm instrumentation with
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Dinglong M. Ma, Julien Bec, Diego R. Yankelevich, Dimitris S. Gorpas, Hussain Fatakdawala, Laura Marcu, "Rotational multispectral fluorescence lifetime imaging and intravascular ultrasound: bimodal system for intravascular applications," Journal of Biomedical Optics 19(6), 066004 (4 June 2014). http://dx.doi.org/10.1117/1.JBO.19.6.066004
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KEYWORDS
Intravascular ultrasound

Luminescence

Imaging systems

Arteries

Data acquisition

Signal to noise ratio

Fiber optics

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