26 June 2014 Identification of dirty necrosis in colorectal carcinoma based on multiphoton microscopy
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J. of Biomedical Optics, 19(6), 066008 (2014). doi:10.1117/1.JBO.19.6.066008
Abstract
Dirty necrosis within glandular lumina is often considered as a characteristic of colorectal carcinomas (CRCs) that is a diagnostically useful feature of CRCs with DNA microsatellite instability (MSI). Multiphoton microscopy (MPM), which is based on the second-harmonic generation and two-photon excited fluorescence signals, was used to identify dirty necrosis. Our results demonstrated that MPM has the ability to exhibit the microstructure of dirty necrosis and the signal intensity as well as an emission spectrum that can help to differentiate dirty necrosis from cancer cells. These findings indicate that MPM may be helpful in distinguishing MSI colorectal carcinoma via the identification of dirty necrosis.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Lianhuang Li, Weizhong Jiang, Yinghong Yang, Zhifen Chen, Changyin Feng, Hongsheng Li, Guoxian Guan, Jianxin Chen, "Identification of dirty necrosis in colorectal carcinoma based on multiphoton microscopy," Journal of Biomedical Optics 19(6), 066008 (26 June 2014). http://dx.doi.org/10.1117/1.JBO.19.6.066008
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KEYWORDS
Multispectral imaging

Second-harmonic generation

Tumors

Collagen

Tissues

Multiphoton microscopy

Colorectal cancer

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