26 June 2014 Identification of dirty necrosis in colorectal carcinoma based on multiphoton microscopy
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Dirty necrosis within glandular lumina is often considered as a characteristic of colorectal carcinomas (CRCs) that is a diagnostically useful feature of CRCs with DNA microsatellite instability (MSI). Multiphoton microscopy (MPM), which is based on the second-harmonic generation and two-photon excited fluorescence signals, was used to identify dirty necrosis. Our results demonstrated that MPM has the ability to exhibit the microstructure of dirty necrosis and the signal intensity as well as an emission spectrum that can help to differentiate dirty necrosis from cancer cells. These findings indicate that MPM may be helpful in distinguishing MSI colorectal carcinoma via the identification of dirty necrosis.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Lianhuang Li, Lianhuang Li, Weizhong Jiang, Weizhong Jiang, Yinghong Yang, Yinghong Yang, Zhifen Chen, Zhifen Chen, Changyin Feng, Changyin Feng, Hongsheng Li, Hongsheng Li, Guoxian Guan, Guoxian Guan, Jianxin Chen, Jianxin Chen, } "Identification of dirty necrosis in colorectal carcinoma based on multiphoton microscopy," Journal of Biomedical Optics 19(6), 066008 (26 June 2014). https://doi.org/10.1117/1.JBO.19.6.066008 . Submission:

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