Noninvasive and label-free determination of virus infected cells by Raman spectroscopy

Kamila Moor; Kiyoshi Ohtani M.D.; Diyas Myrzakozha; Orik Zhanserkenova; Bibin B. Andriana; Hidetoshi Sato

doi:10.1117/1.JBO.19.6.067003

4 June 2014 Noninvasive and label-free determination of virus infected cells by Raman spectroscopy

Kamila Moor, Kiyoshi Ohtani M.D., Diyas Myrzakozha, Orik Zhanserkenova, Bibin B. Andriana, Hidetoshi Sato

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Journal of Biomedical Optics, Vol. 19, Issue 6, 067003 (June 2014). https://doi.org/10.1117/1.JBO.19.6.067003

Abstract

The present study demonstrates that Raman spectroscopy is a powerful tool for the detection of virus-infected cells. Adenovirus infection of human embryonic kidney 293 cells was successfully detected at 12, 24, and 48 h after initiating the infection. The score plot of principal component analysis discriminated the spectra of the infected cells from those of the control cells. The viral infection was confirmed by the conventional immunostaining method performed 24 h after the infection. The newly developed method provides a fast and label-free means for the detection of virus-infected cells.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Kamila Moor, Kiyoshi Ohtani M.D., Diyas Myrzakozha, Orik Zhanserkenova, Bibin B. Andriana, and Hidetoshi Sato "Noninvasive and label-free determination of virus infected cells by Raman spectroscopy," Journal of Biomedical Optics 19(6), 067003 (4 June 2014). https://doi.org/10.1117/1.JBO.19.6.067003

Published: 4 June 2014

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