20 June 2014 Electron multiplying charge-coupled device-based fluorescence cross-correlation spectroscopy for blood velocimetry on zebrafish embryos
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Abstract
Biomedical issues in vasculogenesis and cardiogenesis require methods to follow hemodynamics with high spatial (micrometers) and time (milliseconds) resolution. At the same time, we need to follow relevant morphogenetic processes on large fields of view. Fluorescence cross-correlation spectroscopy coupled to scanning or wide-field microscopy meets these needs but has limited flexibility in the excitation pattern. To overcome this limitation, we develop here a two-photon two-spots setup coupled to an all-reflective near-infrared (NIR) optimized scanning system and to an electron multiplying charge-coupled device. Two NIR laser spots are spaced at adjustable micron-size distances (1 to 50
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Paolo Pozzi, Paolo Pozzi, Laura Sironi, Laura Sironi, Laura D’Alfonso, Laura D’Alfonso, Margaux Bouzin, Margaux Bouzin, Maddalena Collini, Maddalena Collini, Giuseppe Chirico, Giuseppe Chirico, Piersandro Pallavicini, Piersandro Pallavicini, Franco Cotelli, Franco Cotelli, Efrem A. Foglia, Efrem A. Foglia, } "Electron multiplying charge-coupled device-based fluorescence cross-correlation spectroscopy for blood velocimetry on zebrafish embryos," Journal of Biomedical Optics 19(6), 067007 (20 June 2014). https://doi.org/10.1117/1.JBO.19.6.067007 . Submission:
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