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12 March 2014 Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring
Haiyan Xie, Zhiyuan Xie, Monirehalsadat Mousavi, Niels Bendsoe M.D., Mikkel S. Brydegaard, Johan Axelsson, Stefan Andersson-Engels
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Journal of Biomedical Optics, Vol. 19, Issue 7, 071408 (March 2014). https://doi.org/10.1117/1.JBO.19.7.071408
Abstract
Optical techniques for tissue diagnostics currently are experiencing tremendous growth in biomedical applications, mainly due to their noninvasive, inexpensive, and real-time functionality. Here, we demonstrate a hand-held fiber optic probe instrument based on fluorescence/reflectance spectroscopy for precise tumor delineation. It is mainly aimed for brain tumor resection guidance with clinical adaptation to minimize the disruption of the standard surgical workflow and is meant as a complement to the state-of-the-art fluorescence surgical microscopy technique. Multiple light sources with fast pulse modulation and detection enable precise quantification of protoporphyrin IX (PpIX), tissue optical properties, and ambient light suppression. Laboratory measurements show the system is insensitive to strong ambient light. Validation measurements of tissue phantoms using nonlinear least squares support vector machines (LS-SVM) regression analysis demonstrate an error of <5% for PpIX concentration ranging from 400 to 1000 nM, even in the presence of large variations in phantom optical properties. The mean error is 3% for reduced scattering coefficient and 5% for blood concentration. Diagnostic precision of 100% was obtained by LS-SVM classification for in vivo skin tumors with topically applied 5-aminolevulinic acid during photodynamic therapy. The probe could easily be generalized to other tissue types and fluorophores for therapy guidance and monitoring.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Haiyan Xie, Zhiyuan Xie, Monirehalsadat Mousavi, Niels Bendsoe M.D., Mikkel S. Brydegaard, Johan Axelsson, and Stefan Andersson-Engels "Design and validation of a fiber optic point probe instrument for therapy guidance and monitoring," Journal of Biomedical Optics 19(7), 071408 (12 March 2014). https://doi.org/10.1117/1.JBO.19.7.071408
Published: 12 March 2014
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Cited by 16 scholarly publications.
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KEYWORDS
Tissue optics
Luminescence
Tissues
Tumors
Fiber optics
Blood
Light emitting diodes
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