15 July 2014 Target-to-background enhancement in multispectral endoscopy with background autofluorescence mitigation for quantitative molecular imaging
Author Affiliations +
Abstract
Fluorescence molecular imaging with exogenous probes improves specificity for the detection of diseased tissues by targeting unambiguous molecular signatures. Additionally, increased diagnostic sensitivity is expected with the application of multiple molecular probes. We developed a real-time multispectral fluorescence-reflectance scanning fiber endoscope (SFE) for wide-field molecular imaging of fluorescent dye-labeled molecular probes at nanomolar detection levels. Concurrent multichannel imaging with the wide-field SFE also allows for real-time mitigation of the background autofluorescence (AF) signal, especially when fluorescein, a U.S. Food and Drug Administration approved dye, is used as the target fluorophore. Quantitative tissue AF was measured for the
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Chenying Yang, Chenying Yang, Vivian W. Hou, Vivian W. Hou, Emily J. Girard, Emily J. Girard, Leonard Y. Nelson, Leonard Y. Nelson, Eric J. Seibel, Eric J. Seibel, } "Target-to-background enhancement in multispectral endoscopy with background autofluorescence mitigation for quantitative molecular imaging," Journal of Biomedical Optics 19(7), 076014 (15 July 2014). https://doi.org/10.1117/1.JBO.19.7.076014 . Submission:
JOURNAL ARTICLE
16 PAGES


SHARE
Back to Top