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7 August 2014 Label-free photoacoustic nanoscopy
Amos Danielli, Konstantin I. Maslov, Alejandro Garcia-Uribe, Amy M. Winkler, Chiye Li, Lidai Wang, Yun Chen, Gerald W. Dorn II, Lihong V. Wang
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Journal of Biomedical Optics, Vol. 19, Issue 8, 086006 (August 2014). https://doi.org/10.1117/1.JBO.19.8.086006
Abstract
Super-resolution microscopy techniques—capable of overcoming the diffraction limit of light—have opened new opportunities to explore subcellular structures and dynamics not resolvable in conventional far-field microscopy. However, relying on staining with exogenous fluorescent markers, these techniques can sometimes introduce undesired artifacts to the image, mainly due to large tagging agent sizes and insufficient or variable labeling densities. By contrast, the use of endogenous pigments allows imaging of the intrinsic structures of biological samples with unaltered molecular constituents. Here, we report label-free photoacoustic (PA) nanoscopy, which is exquisitely sensitive to optical absorption, with an 88 nm resolution. At each scanning position, multiple PA signals are successively excited with increasing laser pulse energy. Because of optical saturation or nonlinear thermal expansion, the PA amplitude depends on the nonlinear incident optical fluence. The high-order dependence, quantified by polynomial fitting, provides super-resolution imaging with optical sectioning. PA nanoscopy is capable of super-resolution imaging of either fluorescent or nonfluorescent molecules.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Amos Danielli, Konstantin I. Maslov, Alejandro Garcia-Uribe, Amy M. Winkler, Chiye Li, Lidai Wang, Yun Chen, Gerald W. Dorn II, and Lihong V. Wang "Label-free photoacoustic nanoscopy," Journal of Biomedical Optics 19(8), 086006 (7 August 2014). https://doi.org/10.1117/1.JBO.19.8.086006
Published: 7 August 2014
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Cited by 130 scholarly publications and 8 patents.
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KEYWORDS
Super resolution microscopy
Nonlinear optics
Gold
Photoacoustic spectroscopy
Absorption
Nanoparticles
Video
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