20 August 2014 Identification of kidney tumor tissue by infrared spectroscopy of extracellular matrix
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Abstract
Fourier transform infrared (FT-IR) spectroscopy was applied to characterize the extracellular matrix (ECM) of kidney tumor tissue and normal kidney tissue. Freshly resected tissue samples from 31 patients were pressed on a CaF 2 substrate. FT-IR spectra obtained from ECM of tumor tissue exhibit stronger absorption bands in the spectral region from 1000 to 1200  cm −1 and around 1750  cm −1 than those obtained from normal tissue. It is likely that the spectra of ECM of kidney tumor tissue with large increases in the intensities of these bands represent a higher concentration of fatty acids and glycerol. Amide I and amide II bands are stronger in the spectra of ECM from normal tissue, indicating a higher level of proteins. Our results suggest that FT-IR spectroscopy of the ECM is an innovative emerging technology for real-time intraoperative tumor diagnosis, which may improve margin clearance in renal cancer surgery.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Vidita Urboniene, Vidita Urboniene, Milda Pucetaite, Milda Pucetaite, Feliksas Jankevicius, Feliksas Jankevicius, Arunas Zelvys, Arunas Zelvys, Valdas Sablinskas, Valdas Sablinskas, Gerald Steiner, Gerald Steiner, } "Identification of kidney tumor tissue by infrared spectroscopy of extracellular matrix," Journal of Biomedical Optics 19(8), 087005 (20 August 2014). https://doi.org/10.1117/1.JBO.19.8.087005 . Submission:
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