Comparative evaluation of methylene blue and demeclocycline for enhancing optical contrast of gliomas in optical images

Dennis J. Wirth; Matija Snuderl; William Curry; Anna Yaroslavsky

doi:10.1117/1.JBO.19.9.090504

19 September 2014 Comparative evaluation of methylene blue and demeclocycline for enhancing optical contrast of gliomas in optical images

Dennis J. Wirth, Matija Snuderl, William Curry, Anna Yaroslavsky

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Journal of Biomedical Optics, Vol. 19, Issue 9, 090504 (September 2014). https://doi.org/10.1117/1.JBO.19.9.090504

Abstract

Contrast agents have shown to be useful in the detection of cancers. The goal of this study was to compare enhancement of brain cancer contrast using reflectance and fluorescence confocal imaging of two fluorophores, methylene blue (MB) and demeclocycline (DMN). MB absorbs light in the red spectral range and fluoresces in the near-infrared. It is safe for in vivo staining of human skin and breast tissue. However, its safety for staining human brain is questionable. Thus, DMN, which absorbs light in the violet spectral range and fluoresces between 470 and 570 nm, could provide a safer alternative to MB. Fresh human gliomas, obtained from surgeries, were cut in half and stained with aqueous solutions of MB and DMN, respectively. Stained tissues were imaged using multimodal confocal microscopy. Resulting reflectance and fluorescence optical images were compared with hematoxylin and eosin histopathology, processed from each imaged tissue. Results indicate that images of tissues stained with either stain exhibit comparable contrast and resolution of morphological detail. Further studies are required to establish the safety and efficacy of these contrast agents for use in human brain.

CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Dennis J. Wirth, Matija Snuderl, William Curry, and Anna Yaroslavsky "Comparative evaluation of methylene blue and demeclocycline for enhancing optical contrast of gliomas in optical images," Journal of Biomedical Optics 19(9), 090504 (19 September 2014). https://doi.org/10.1117/1.JBO.19.9.090504

Published: 19 September 2014

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