15 September 2014 Microvascular quantification based on contour-scanning photoacoustic microscopy
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Accurate quantification of microvasculature remains of interest in fundamental pathophysiological studies and clinical trials. Current photoacoustic microscopy can noninvasively quantify properties of the microvasculature, including vessel density and diameter, with a high spatial resolution. However, the depth range of focus (i.e., focal zone) of optical-resolution photoacoustic microscopy (OR-PAM) is often insufficient to encompass the depth variations of features of interest—such as blood vessels—due to uneven tissue surfaces. Thus, time-consuming image acquisitions at multiple different focal planes are required to maintain the region of interest in the focal zone. We have developed continuous three-dimensional motorized contour-scanning OR-PAM, which enables real-time adjustment of the focal plane to track the vessels’ profile. We have experimentally demonstrated that contour scanning improves the signal-to-noise ratio of conventional OR-PAM by as much as 41% and shortens the image acquisition time by 3.2 times. Moreover, contour-scanning OR-PAM more accurately quantifies vessel density and diameter, and has been applied to studying tumors with uneven surfaces.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Chenghung Yeh, Chenghung Yeh, Brian T. Soetikno, Brian T. Soetikno, Song Hu, Song Hu, Konstantin I. Maslov, Konstantin I. Maslov, Lihong V. Wang, Lihong V. Wang, } "Microvascular quantification based on contour-scanning photoacoustic microscopy," Journal of Biomedical Optics 19(9), 096011 (15 September 2014). https://doi.org/10.1117/1.JBO.19.9.096011 . Submission:

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