Thermal evaluation of laser exposures in an in vitro retinal model by microthermal sensing

Tae-Youl Choi; Michael L. Denton; Gary D. Noojin; Larry E. Estlack; Ramesh L. Shrestha; Benjamin A. Rockwell; Robert J. Thomas; Dongsik Kim

doi:10.1117/1.JBO.19.9.097003

15 September 2014 Thermal evaluation of laser exposures in an in vitro retinal model by microthermal sensing

Tae-Youl Choi; Michael L. Denton; Gary D. Noojin; Larry E. Estlack; Ramesh L. Shrestha; Benjamin A. Rockwell; Robert J. Thomas; Dongsik Kim;

Author Affiliations +

J. of Biomedical Optics, 19(9), 097003 (2014). doi:10.1117/1.JBO.19.9.097003

Abstract

A temperature detection system using a micropipette thermocouple sensor was developed for use within mammalian cells during laser exposure with an 8.6-μm beam at 532 nm. We have demonstrated the capability of measuring temperatures at a single-cell level in the microscale range by inserting micropipette-based thermal sensors of size ranging from 2 to 4 μm into the membrane of a live retinal pigment epithelium (RPE) cell subjected to a laser beam. We setup the treatment groups of 532-nm laser-irradiated single RPE cell and in situ temperature recordings were made over time. Thermal profiles are given for representative cells experiencing damage resulting from exposures of 0.2 to 2 s. The measured maximum temperature rise for each cell ranges from 39 to 73°C; the RPE cells showed a signature of death for all the cases reported herein. In order to check the cell viability, real-time fluorescence microscopy was used to identify the transition of pigmented RPE cells between viable and damaged states due to laser exposure.

Citation Download Citation

Tae-Youl Choi, Michael L. Denton, Gary D. Noojin, Larry E. Estlack, Ramesh L. Shrestha, Benjamin A. Rockwell, Robert J. Thomas, Dongsik Kim, "Thermal evaluation of laser exposures in an in vitro retinal model by microthermal sensing," Journal of Biomedical Optics 19(9), 097003 (15 September 2014). https://doi.org/10.1117/1.JBO.19.9.097003

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