15 September 2014 Thermal evaluation of laser exposures in an <italic<in vitro</italic< retinal model by microthermal sensing
Author Affiliations +
J. of Biomedical Optics, 19(9), 097003 (2014). doi:10.1117/1.JBO.19.9.097003
A temperature detection system using a micropipette thermocouple sensor was developed for use within mammalian cells during laser exposure with an 8.6-μm beam at 532 nm. We have demonstrated the capability of measuring temperatures at a single-cell level in the microscale range by inserting micropipette-based thermal sensors of size ranging from 2 to 4  μm into the membrane of a live retinal pigment epithelium (RPE) cell subjected to a laser beam. We setup the treatment groups of 532-nm laser-irradiated single RPE cell and in situ temperature recordings were made over time. Thermal profiles are given for representative cells experiencing damage resulting from exposures of 0.2 to 2 s. The measured maximum temperature rise for each cell ranges from 39 to 73°C; the RPE cells showed a signature of death for all the cases reported herein. In order to check the cell viability, real-time fluorescence microscopy was used to identify the transition of pigmented RPE cells between viable and damaged states due to laser exposure.
© 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)
Tae-Youl Choi, Michael L. Denton, Gary D. Noojin, Larry E. Estlack, Ramesh L. Shrestha, Benjamin A. Rockwell, Robert J. Thomas, Dongsik Kim, "Thermal evaluation of laser exposures in an <italic<in vitro</italic< retinal model by microthermal sensing," Journal of Biomedical Optics 19(9), 097003 (15 September 2014). http://dx.doi.org/10.1117/1.JBO.19.9.097003

Temperature metrology



In vitro testing

Thermal modeling

Thermal sensing

Infrared sensors

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