1 October 1997 Ratio-based decisions and the quantitative analysis of cDNA microarray images
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Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image segmentation is provided to identify cDNA target sites and a hypothesis test and confidence interval is developed to quantify the significance of observed differences in expression ratios. In particular, the probability density of the ratio and the maximum-likelihood estimator for the distribution are derived, and an iterative procedure for signal calibration is developed.
Yidong Chen, Edward R. Dougherty, Michael L. Bittner, "Ratio-based decisions and the quantitative analysis of cDNA microarray images," Journal of Biomedical Optics 2(4), (1 October 1997). https://doi.org/10.1117/12.281504

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