1 October 1997 Ratio-based decisions and the quantitative analysis of cDNA microarray images
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Abstract
Gene expression can be quantitatively analyzed by hybridizing fluor-tagged mRNA to targets on a cDNA microarray. Comparison of gene expression levels arising from cohybridized samples is achieved by taking ratios of average expression levels for individual genes. A novel method of image segmentation is provided to identify cDNA target sites and a hypothesis test and confidence interval is developed to quantify the significance of observed differences in expression ratios. In particular, the probability density of the ratio and the maximum-likelihood estimator for the distribution are derived, and an iterative procedure for signal calibration is developed.
Yidong Chen, Yidong Chen, Edward R. Dougherty, Edward R. Dougherty, Michael L. Bittner, Michael L. Bittner, } "Ratio-based decisions and the quantitative analysis of cDNA microarray images," Journal of Biomedical Optics 2(4), (1 October 1997). https://doi.org/10.1117/12.281504 . Submission:
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