13 August 2015 Static laser speckle contrast analysis for noninvasive burn diagnosis using a camera-phone imager
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J. of Biomedical Optics, 20(8), 086009 (2015). doi:10.1117/1.JBO.20.8.086009
Laser speckle contrast analysis (LASCA) is an established optical technique for accurate widefield visualization of relative blood perfusion when no or minimal scattering from static tissue elements is present, as demonstrated, for example, in LASCA imaging of the exposed cortex. However, when LASCA is applied to diagnosis of burn wounds, light is backscattered from both moving blood and static burn scatterers, and thus the spatial speckle contrast includes both perfusion and nonperfusion components and cannot be straightforwardly associated to blood flow. We extract from speckle contrast images of burn wounds the nonperfusion (static) component and discover that it conveys useful information on the ratio of static-to-dynamic scattering composition of the wound, enabling identification of burns of different depth in a porcine model <italic<in vivo</italic< within the first 48 h postburn. Our findings suggest that relative changes in the static-to-dynamic scattering composition of burns can dominate relative changes in blood flow for burns of different severity. Unlike conventional LASCA systems that employ scientific or industrial-grade cameras, our LASCA system is realized here using a camera phone, showing the potential to enable LASCA-based burn diagnosis with a simple imager.
Udi Willenz, Sigal Ragol, Itay Remer, Yaron Shoham, Sivan Hazan, Igor Sinelnikov, Vladimir Dronov, Lior Rosenberg, Alberto Bilenca, "Static laser speckle contrast analysis for noninvasive burn diagnosis using a camera-phone imager," Journal of Biomedical Optics 20(8), 086009 (13 August 2015). http://dx.doi.org/10.1117/1.JBO.20.8.086009




Imaging systems


Laser scattering

Statistical analysis

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