27 October 2015 Mechanism of two-photon excited hemoglobin fluorescence emission
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Hemoglobin, one of the most important proteins in the human body, is composed of “heme” groups (iron-containing rings) and “globins” (proteins). We investigate the two-photon excited fluorescence of hemoglobin and its subunit components (heme and globin). We measure the hemoglobin fluorescence lifetime by using a streak camera of ps resolution and confirm that its lifetime is in femtosecond scale. In the study of the fluorescence properties of heme and globin, the experimental results reveal that heme is the sole fluorophore of hemoglobin. Hemoglobin fluorescence can be effectively excited only via two-photon process, because heme has a centrosymmetric molecular structure and two-photon allowed transition is forbidden for single-photon process and vice versa due to the Laporte parity selection rule.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE)
Qiqi Sun, Qiqi Sun, Wei Zheng, Wei Zheng, Jiannong Wang, Jiannong Wang, Yi Luo, Yi Luo, Jianan Y. Qu, Jianan Y. Qu, } "Mechanism of two-photon excited hemoglobin fluorescence emission," Journal of Biomedical Optics 20(10), 105014 (27 October 2015). https://doi.org/10.1117/1.JBO.20.10.105014 . Submission:

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