Noncontact photoacoustic tomography of in vivo chicken chorioallantoic membrane based on all-fiber heterodyne interferometry

Jonghyun Eom; Seong Jun Park; Byeong Ha Lee

doi:10.1117/1.JBO.20.10.106007

16 October 2015 Noncontact photoacoustic tomography of in vivo chicken chorioallantoic membrane based on all-fiber heterodyne interferometry

Jonghyun Eom, Seong Jun Park, Byeong Ha Lee

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Journal of Biomedical Optics, Vol. 20, Issue 10, 106007 (October 2015). https://doi.org/10.1117/1.JBO.20.10.106007

Abstract

We present three-dimensional (3-D) in vivo photoacoustic (PA) images of the blood vasculature of a chicken chorioallantoic membrane (CAM) obtained by using a fiber-based noncontact PA tomography system. With a fiber-optic heterodyne interferometer, the system measures the surface displacement of a sample, induced by the PA wave, which overcomes the disadvantage of physical-contact of ultrasonic transducer in a conventional system. The performance of an implemented system is analyzed and its capability of in vivo 3-D bioimaging is presented. At a depth of 2.5 mm in a phantom experiment, the lateral and axial resolutions were measured as 100 and 30 μm, respectively. The lateral resolution became doubled at a depth of 7.0 mm; however, interestingly, the axial resolution was not noticeably deteriorated with the depth. With the CAM experiment, performed under the American National Standards Institute laser safety standard condition, blood vessel structures placed as deep as 3.5 mm were clearly recognized.

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Jonghyun Eom, Seong Jun Park, and Byeong Ha Lee "Noncontact photoacoustic tomography of in vivo chicken chorioallantoic membrane based on all-fiber heterodyne interferometry," Journal of Biomedical Optics 20(10), 106007 (16 October 2015). https://doi.org/10.1117/1.JBO.20.10.106007

Published: 16 October 2015

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