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28 October 2015 Video-rate in vivo fluorescence imaging with a line-scanned dual-axis confocal microscope
Ye Chen, Danni Wang, Altaz Khan, Yu Wang, Sabine Borwege, Nader Sanai, Jonathan T. C. Liu
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Journal of Biomedical Optics, Vol. 20, Issue 10, 106011 (October 2015). https://doi.org/10.1117/1.JBO.20.10.106011
Abstract
Video-rate optical-sectioning microscopy of living organisms would allow for the investigation of dynamic biological processes and would also reduce motion artifacts, especially for in vivo imaging applications. Previous feasibility studies, with a slow stage-scanned line-scanned dual-axis confocal (LS-DAC) microscope, have demonstrated that LS-DAC microscopy is capable of imaging tissues with subcellular resolution and high contrast at moderate depths of up to several hundred microns. However, the sensitivity and performance of a video-rate LS-DAC imaging system, with low-numerical aperture optics, have yet to be demonstrated. Here, we report on the construction and validation of a video-rate LS-DAC system that possesses sufficient sensitivity to visualize fluorescent contrast agents that are topically applied or systemically delivered in animal and human tissues. We present images of murine oral mucosa that are topically stained with methylene blue, and images of protoporphyrin IX-expressing brain tumor from glioma patients that have been administered 5-aminolevulinic acid prior to surgery. In addition, we demonstrate in vivo fluorescence imaging of red blood cells trafficking within the capillaries of a mouse ear, at frame rates of up to 30 fps. These results can serve as a benchmark for miniature in vivo microscopy devices under development.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Ye Chen, Danni Wang, Altaz Khan, Yu Wang, Sabine Borwege, Nader Sanai, and Jonathan T. C. Liu "Video-rate in vivo fluorescence imaging with a line-scanned dual-axis confocal microscope," Journal of Biomedical Optics 20(10), 106011 (28 October 2015). https://doi.org/10.1117/1.JBO.20.10.106011
Published: 28 October 2015
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Cited by 12 scholarly publications and 1 patent.
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KEYWORDS
In vivo imaging
Microscopes
Confocal microscopy
Luminescence
Tissues
Blood
Microscopy
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